Cytotoxicity of HBD3 for dendritic cells, normal human epidermal keratinocytes, hTERT keratinocytes, and primary oral gingival epithelial keratinocytes in cell culture conditions

Differential cytotoxicity of long-chain bases for human oral gingival epithelial keratinocytes, oral fibroblasts, and dendritic cells

Long-chain bases are present in the oral cavity. Previously we determined that sphingosine, dihydrosphingosine, and phytosphingosine have potent antimicrobial activity against oral pathogens. Here, we determined the cytotoxicities of long-chain bases for oral cells, an important step in considering their potential as antimicrobial agents for oral infections. This information would clearly help ...

Cultured Normal Human Epidermal Keratinocytes

H]glucosamine has been used to label metabolically keratinocyte cell-surface glycoconjugates. The major labelled bands identified on sodium dodecyl sulphate/polyacrylamide gels had apparent molecular weights of greater than 250000, and 150000-80000. Most of these components were trypsin-sensitive, indicating that the label was protein-bound. Some of the labelled components were shown to be prot...

Isolation and in vitro cultivation of human skin Keratinocytes and preparation of epidermal sheet

Epithelial Phenotype in Human Keratinocytes

In the current study, we investigated the effects of p63 modulation in epithelial plasticity in human keratinocytes. p63 isoforms ΔNp63α, ΔNp63β, and ΔNp63γ were ectopically expressed in normal human epidermal keratinocytes (NHEK). Epithelial or mesenchymal state was determined by morphological changes and altered expression of various markers, e.g., fibronectin (FN), ECadherin (E-Cad), and ker...

DIFFERENTIATION OF HUMAN OVARIAN FOLLICULAR GRANULOSA CELLS INTO KERATINOCYTES

Background & Aims: Stem cells are undifferentiated cells and are found in different tissues. These cells have capacity of self-renewal and differentiation into other lineages. Granulosa cells (GCs) are the multipotent stem cells. In the present research we evaluated the differentiation potential of GCs into keratinocytes. Material & Methods: GCs were cultured after enzymatic isolation from ova...